ayy lmao i made a blog


help pls

Comments

Post a Comment

Popular posts from this blog

Lab 2: Media Prep and Autoclave, Plate Pouring

Image
Lab 2: Media Prep and Autoclave, Plate Pouring (Photo: Chester) Introduction     The purpose of this lab will ultimately be to prepare and pour plates of two different medias in preparation for later labs.  Pouring the appropriate amount of media into each petri dish will be the biggest issue and it is predicted that the technique will get better as more plates are poured.     While there are many different types of media used in microbiology labs, one of the most common is Luria-Bertani (LB).  Due to its richness in nutrients, it provides good food for bacteria and is simple to make. In addition to its prime conditions for a pure culture, it provides enough food so a culture may grow quickly.     While LB is typically used to grow Escherichia coli , it is a common media in every lab.  The most common recipe is: combine 10g of tryptone, 5 g of yeast extract, 10 g of NaCl and 1 liter of distilled water; adjust the pH to 7.0 with 1 N Na...
Read more

Lab 4: Using the Spectrophotometer to Measure the Rate of Bacterial Cell Division: A Bacterial Population Growth Curve

Image
Lab 4:  Using the Spectrophotometer to Measure the Rate of Bacterial Cell Division: A Bacterial Population Growth Curve (Photo:Miamioh) Introduction     The purpose of this lab will ultimately be to observe the growth of an Escherichia coli population in its lag phase, its exponential growth phase and its stationary phase.  Each group will measure the turbidity of different E. coli samples all taken at different times to get a wide range of measurements that will then construct a growth graph.  The biggest issue will most likely be the counting of the hemocytometerns that contain the E. coli .     While it is different for every bacteria, E. coli has a doubling time as short as 20 minutes. However, once they are placed in a growth medium (in this case LB broth), they will take some getting used to their environment. This period of adjustment is called the lag phase and will seen as the initial flatline on the growth curve graph.  Once th...
Read more

Lab 6: Coliform Determination by the Membrane Filter Technique

Image
Lab 6:  Lab Coliform Determination by the Membrane Filter Technique (Photo: Steve Nease) Introduction     The purpose of this lab will ultimately be to familiarize ourselves with counting coliform microorganism colonies as well as another plating method called the membrane filter technique.     The total viable count is based upon the ability of a microorganism placed in a nutrient environment to grow.  While individual microorganisms cannot be seen with the naked eye, they can grow in colonies which are visible to any plain Jane.  Counting the colonies gives an indication of the number of viable colony forming units (CFU) present in the original sample.  However, the appropriate growth medium must be chosen first to get to the counting phase.  This part is crucial because the accuracy will be best if the chosen medium is as similar as possible to the sample environment.     The membrane filter technique uses the semi pe...
Read more