Lab 1: Working with a Pipette
Lab 1: Working with a Pipette (Photo: Ralo) Introduction As more classes become integrated with laboratory techniques, the proper handling and care of different materials is crucial to such success. The objective of this lab was to learn how to correctly and accurately use micropipettors to measure small volumes of liquid. The FGCU lab is equipped with the Eppendorf pipettes, particularly the 10-100 μL (yellow pipette) and the 100-1000 μL (blue pipette) versions. Any measurement above or below the defined range (listed on the top of the device) does not guarantee accuracy. By twisting the grooved wheel on top of the pipette, the desired measurement can be increased or decreased and read on the side of the device. Each pipette is to be used with a particular disposable tip in the sanitized plastic racks: yellow tip=yellow (100) pipette, blue tip=blue (1000) pipette. Tips are attached by opening the plastic rack, pressing the pipette down on to a tip to attach and qui
